JM Bioscience, a Fetal Bovine Serum (FBS) supplier serving the research needs of the life science community

Tel.: (760) 300-5534 Fax: (619) 342-1691 Email: help@jmbioscience.com

FBS usage guidelines

Storage Upon receipt, frozen FBS should be immediately moved into a -20C (or below) freezer and protected from light; it should not be allowed to thaw until ready for use. FBS should last more than five years under these conditions. We do not recommend that FBS be defrosted and refrozen more than once.
Thawing We recommend rapid thawing: warm the frozen FBS at room temperature for 10 minutes, and then place into a 37C water-bath. Rapid thawing limits high salt concentrations, which can potentially degrade serum proteins. Gently rotate-do not shake as this can cause detrimental foaming. FBS should be removed from the water bath when fully thawed, and not be allowed to warm to ambient temperature. When thawed, FBS is normally a clear amber solution; however, there may be small amounts of crystalline precipitates (salts, lipids, & proteins), which will not affect performance. Filtration of these precipitates is usually not necessary or recommended. Thawed FBS should be stored at 4C and used with 2-3 weeks.
Usage FBS is typically used at 1-20% (vol) for media preparation in tissue culture, with 10% (vol) being the most common.
Batch Variation As FBS is a complex animal product, there can be significant batch-to-batch variation. While we performance-test each batch on several common cell lines, we recommend that end-users batch test sera on their particular cell line(s).
 
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